Engineering enzymes
نویسندگان
چکیده
منابع مشابه
Engineering enzymes for biocatalysis.
Protein engineering techniques have been available for over two decades beginning with the development of methods for genetic engineering. Since that time, the engineering of enzymes has advanced rapidly along with a revolution in the range and efficiency of new techniques and strategies for designing and evolving proteins in the laboratory. While recent advances in high-throughput screening te...
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Light control over enzyme function represents a novel and exciting field of biocatalysis research. Blue-light photoreceptors of the Light, Oxygen, Voltage (LOV) family have recently been investigated for their applicability as photoactive switches. We discuss here the primary photochemical events leading to light activation of LOV domains as well as the proposed signal propagation mechanism to ...
متن کاملComputational tools for designing and engineering enzymes.
Protein engineering strategies aimed at constructing enzymes with novel or improved activities, specificities, and stabilities greatly benefit from in silico methods. Computational methods can be principally grouped into three main categories: bioinformatics; molecular modelling; and de novo design. Particularly de novo protein design is experiencing rapid development, resulting in more robust ...
متن کاملEngineering of Enzymes for Selective Catalysis
Naturally occurring enzymes are truly remarkable catalysts. However, many are not suitable for practical synthetic chemistry because of poor substrate or product selectivity. This review highlights recent advances in engineering enzymes for selective catalysis. Selected topics include altering substrate specificity, altering substrate and product selectivity, engineering enzymes that catalyze c...
متن کاملSpatial organization of enzymes for metabolic engineering.
As synthetic pathways built from exogenous enzymes become more complicated, the probability of encountering undesired interactions with host organisms increases, thereby lowering product titer. An emerging strategy to combat this problem is to spatially organize pathway enzymes into multi-protein complexes, where high local concentrations of enzymes and metabolites may enhance flux and limit pr...
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ژورنال
عنوان ژورنال: Faraday Discuss.
سال: 2011
ISSN: 1359-6640,1364-5498
DOI: 10.1039/c005523a